| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0448-25 | 7 days | 25 mg | $220.00 |
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|
Product Overview | |
| Name | Insulin Porcine |
|---|---|
| Description | |
| Insulin Porcine | |
| Accession (Primary) | P01315 |
| Introduction | |
| Internalins are surface proteins located on Listeria monocytogenes. Internalins exist in two known forms, InlA and InlB. Internalins are used by the bacteria to invade mammalian cells via cadherins transmembrane proteins. The precise role of the Internalin proteins and their invasiveness in vivo is not entirely understood. However, in cultured cells, InlA is needed to facilitate Listeria entry into human epithelial cells. While InlB is required for Listeria internalisation in several other cell types, including hepatocytes, fibroblasts, and epithelioid cells. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Internalin-A is lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB pH7.4 and 300mM NaCl. | |
| Stability | |
| Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 95.0% as determined by SDS-PAGE and HPLC. | |
| Amino acid sequence | |
| MANENNFLEI MSETKEIKIV NIPDNNLKKV LNKSLNKSEN SDLTVKDLES IEYLYGIAEN ISNIEGLEYC KNLKILSLQN NDNSKKENFN TITDLSPLKY LKNLVVLDLR NNKISDLSPL ENLTNLESLR LSGNNISNIS PLNKLESLTT LTLSYNEITD ISTISNLKNL THLALYNNKI EDISSLKENT KLQNLSLGFN KIKDISVLSN LKNLYDLSLE ENNIKSIKSL SNLHKLSNIN LKNNKIEDIS PLETCNDFFE KLILDGNRIS DISSLSKKEI IVCSINNQTI TIKDWKVSDN NSFEIKLPND NLDIKIDNIS ENGTFIDNSI KWTNISPKSK LSFEFKQDID NDIHSIFYSG TVRFKR LEHHHH HH . | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Important note: If the concentration of NaCl is less than 150mM, the protein solution becomes turbid overnight. Please always keep the concentration of NaCl more than 250mM. | |
| Precautions | |
| Insulin Porcine is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P01315 ) | |
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