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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0455-100 | 7 days | 100 µg | $700.00 |
Select product before adding to cart
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| GRF0455-200 | 7 days | 200 µg | $1,000.00 | |||
| GRF0455-1 | 7 days | 1 mg | $2,720.00 |
Product Overview | |
| Name | Leptin Salamander |
|---|---|
| Description | |
| Leptin Salamander Recombinant | |
| Source | |
| Escherichia coli. | |
| Physical Appearance | |
| White lyophilized (freeze-dried) powder. | |
| Formulation | |
| The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. | |
| Stability | |
| Lyophilized Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln. | |
| Biological Activity | |
| Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Human Recombinant in sterile 0.4% NaHCO3, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title: Leptin-induced vascular smooth muscle cell proliferation via regulating cell cycle, activating ERK1/2 and NF-kB Publication: Acta biochimica et biophysica Sinica 42.5 (2010): 325-331. Link: Leptin tA prospec publication | |
| Protein content | |
| Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | |
| Precautions | |
| Leptin Salamander is for research use only and not for use in diagnostic or therapeutic procedures. | |
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ADMEbio Team
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ADMEbio Team
