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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| ABM0292-100 | 7 days | 100 µg | $310.00 |
Select product before adding to cart
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| ABM0292-0.2 | 7 days | 0.2 mg | $580.00 | |||
| ABM0292-0.5 | 7 days | 0.5 mg | $1,040.00 |
Product Overview | |
| Name | GST Monoclonal antibody |
|---|---|
| Description | |
| Glutathione-S-Transferase, Mouse antibody | |
| Immunogen | |
| Anti GST mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant GST purified from E. coli. | |
| Introduction | |
| GST family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called ''GST gene fusion system''. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector''s multiple cloning sites results in expression of a fusion protein - the protein of int | |
| Physical Appearance | |
| Sterile filtered colorless solution. | |
| Formulation | |
| 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | |
| Applications | |
| GST antibody has been tested ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000. | |
| Type | |
| Mouse Antibody Monoclonal. | |
| Clone | |
| P1E5AT. | |
| Ig Subclass | |
| Mouse IgG2b heavy chain and κ light chain. | |
| Purification Method | |
| GST antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | |
| Storage Procedures | |
| For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | |
| Stability / Shelf Life | |
| 12 months at -20°C. 1 month at 4°C. | |
| Precautions | |
| GST Monoclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
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ADMEbio Team
