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KD-Validated Anti-ACADM Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1369
Application
WB, FC, ICC
Uniprot Id
P11310
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ACADM antibody (Cat#ABG1369). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACADM antibody (Cat#ABG1369, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ACADM antibody (Cat#ABG1369). ACADM expression in wild type (WT) and ACADM knockdown (KD) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ACADM antibody (Cat#ABG1369, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ACADM expression in HepG2 cells using anti-ACADM antibody (Cat#ABG1369, 1:2,000). Green, isotype control; red, ACADM.
  • Immunocytochemical staining of HepG2 cells with anti-ACADM antibody (Cat#ABG1369, 1:1,000). Nuclei were stained blue with DAPI; ACADM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1369-100 3 days 100 µL $499.00
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ABG1369-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ACADM Rabbit Monoclonal Antibody
Accession (Primary)P11310
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3145
Immunogen
A synthesized peptide derived from human ACADM
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 47 kDa; observed, 40 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P11310 )

Gene NameACADM
Other Gene Names
ACADM; Acyl-CoA Dehydrogenase Medium Chain 2 3 5; MCAD; ACAD1; Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, C-4 To C-12 Straight Chain; Medium-Chain Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenase, C-4 To C-12 Straight Chain; Medium Chain Acyl-CoA Dehydrogenase; Testicular Tissue Protein Li 7; EC 1.3.99.3; EC 1.3.8.7; EC 1.3.99
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