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KD-Validated Anti-AP2M1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1317
Application
WB, ICC
Uniprot Id
Q96CW1
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-AP2M1 antibody (Cat#ABG1317). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP2M1 antibody (Cat#ABG1317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-AP2M1 antibody (Cat#ABG1317). AP2M1 expression in wild type (WT) and AP2M1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AP2M1 antibody (Cat#ABG1317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of C2C12 cells with AP2M1 antibody (Cat#ABG1317, 1:1,000). Nuclei were stained blue with DAPI; AP2M1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1317-100 3 days 100 µL $499.00
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ABG1317-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AP2M1 Rabbit Monoclonal Antibody
Accession (Primary)Q96CW1
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3220
Immunogen
A synthesized peptide derived from human AP2M1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 50 kDa , observed, 50 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q96CW1 )

Gene NameAP2M1
Other Gene Names
AP2M1; Adaptor Related Protein Complex 2 Subunit Mu 1; CLAPM1; AP50; Clathrin-Associated/Assembly/Adaptor Protein, Medium 1; Clathrin Assembly Protein Complex 2 Mu Medium Chain; Clathrin Assembly Protein Complex 2 Medium Chain; Adaptor Related Protein Complex 2 Mu 1 Subunit; Adaptor-Related Protein Complex 2 Subunit Mu; Plasma Membrane Adaptor AP-2 50 KDa Protein; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Adaptor Complex AP2, Mu Subunit; Adaptor Protein Complex AP-2 Subunit Mu; Clathrin Coat-Associated Protein AP50; Clathrin Coat Assembly Protein AP50; Clathrin Coat Adaptor Protein AP50; AP-2 Complex Subunit Mu; HA2 50 KDA Subunit; AP-2 Mu 2 Chain; Adaptin-Mu2; Mu2; HA2 50 KDa Subunit; AP-2 Mu Chain; KIAA0109; MRD60; MU2
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