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KD-Validated Anti-ARID1A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1318
Application
WB, FC, ICC
Uniprot Id
O14497
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ARID1A antibody (Cat#ABG1318). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARID1A antibody (Cat#ABG1318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ARID1A antibody (Cat#ABG1318). ARID1A expression in wild type (WT) and ARID1A shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARID1A antibody (Cat#ABG1318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ARID1A expression in C2C12 cells using ARID1A antibody (Cat#ABG1318, 1:2000). Green, isotype control; red, ARID1A.
  • Immunocytochemical staining of C2C12 cells with ARID1A antibody (Cat#ABG1318, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1318-100 3 days 100 µL $499.00
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ABG1318-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ARID1A Rabbit Monoclonal Antibody
Accession (Primary)O14497
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3230
Immunogen
A synthesized peptide derived from human ARID1A
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 242 kDa, observed, 270 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O14497 )

Gene NameARID1A
Other Gene Names
ARID1A; AT-Rich Interaction Domain 1A; BAF250; B120; SMARCF1; P270; SWI/SNF-Related, Matrix-Associated, Actin-Dependent Regulator Of Chromatin Subfamily F Member 1; AT-Rich Interactive Domain-Containing Protein 1A; AT Rich Interactive Domain 1A (SWI-Like); ARID Domain-Containing Protein 1A; SWI/SNF Complex Protein P270; BRG1-Associated Factor 250a; SWI-Like Protein; Osa Homolog 1; BAF250a; BAF250A; C1orf4; HOSA1; OSA1; HELD; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily F, Member 1; AT Rich Interactive Domain 1A (SWI- Like); Chromatin Remodeling Factor P250; BRG1-Associated Factor 250; OSA1 Nuclear Protein; Brain Protein 120; C10rf4; C10RF4; C1ORF4; BM029; MRD14; CSS2; ELD
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