Western blotting analysis using anti-Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343). Acetyl-CoA acetyltransferase 1 expression in wild type (WT) and acetyl-CoA acetyltransferase 1 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Acetyl-CoA acetyltransferase 1 expression in HepG2 cells using Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343, 1:2,000). Green, isotype control; red, Acetyl-CoA acetyltransferase 1.

Immunocytochemical staining of HT-1080 cells with Acetyl-CoA acetyltransferase 1 antibody (Cat#ABG2343, 1:1,000). Nuclei were stained blue with DAPI;Acetyl-CoA acetyltransferase 1 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.