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KD-Validated Anti-Acetyl-CoA acyltransfe rase 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1304
Application
WB, FC, ICC
Uniprot Id
P42765
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304). Acetyl-CoA acyltransferase 2 expression in wild type (WT) and Acetyl-CoA acyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Acetyl-CoA acyltransferase 2 expression in C2C12 cells using Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304, 1:2,000). Green, isotype control; red, Acetyl-CoA acyltransferase 2.
  • Immunocytochemical staining of C2C12 cells with Acetyl-CoA acyltransferase 2 antibody (Cat#ABG1304, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1304-100 3 days 100 µL $499.00
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ABG1304-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Acetyl-CoA acyltransfe rase 2 Rabbit Monoclonal Antibody
Accession (Primary)P42765
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2955
Immunogen
A synthesized peptide derived from human ACAA2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 42 kDa , observed, 45 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P42765 )

Gene NameACAA2
Other Gene Names
ACAA2; Acetyl-CoA Acyltransferase 2; DSAEC 2; Mitochondrial 3-Oxoacyl-Coenzyme A Thiolase 2; 3-Ketoacyl-CoA Thiolase, Mitochondrial; Mitochondrial 3-Oxoacyl-CoA Thiolase; Acetyl-Coenzyme A Acyltransferase 2; Acyl-CoA Hydrolase, Mitochondrial; Acetyl-CoA Acetyltransferase; EC 2.3.1.16; T1; Acetyl-CoA Acyltransferase; Beta Ketothiolase; Beta-Ketothiolase; EC 2.3.1.9; EC 3.1.2.-; EC 3.1.2.1; EC 3.1.2.2; EC 2.3.1
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