Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#ABG1362). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#ABG1362, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.

Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#ABG1362). Adenosine deaminase RNA specific expression in wild-type (WT) and adenosine deaminase RNA specific (ADAR) knockdown (KD) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#ABG1362, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#ABG1362, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific.

lmmunocytochemical staining of HepG2 cells with Adenosine deaminase RNA specific antibody (Cat#ABG1362, 1:1,000). Nuclei were stained blue with DAPl; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.