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KD-Validated Anti-Aryl hydrocarbon receptor Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1170
Application
WB, FC, ICC
Uniprot Id
P35869
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Aryl hydrocarbon receptor antibody (Cat#ABG1170). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aryl hydrocarbon receptor antibody (Cat#ABG1170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Aryl hydrocarbon receptor antibody (Cat#ABG1170). Aryl hydrocarbon receptor expression in wild type (WT) and aryl hydrocarbon receptor shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Aryl hydrocarbon receptor antibody (Cat#ABG1170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Aryl hydrocarbon receptor expression in HepG2 cells using Aryl hydrocarbon receptor antibody (Cat#ABG1170, 1:2,000). Green, isotype control; red, Aryl hydrocarbon receptor.
  • Immunocytochemical staining of HepG2 cells with Aryl hydrocarbon receptor antibody (Cat#ABG1170, 1:1,000). Nuclei were stained blue with DAPI; Aryl hydrocarbon receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1170-100 3 days 100 µL $499.00
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ABG1170-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Aryl hydrocarbon receptor Rabbit Monoclonal Antibody
Accession (Primary)P35869
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1635
Immunogen
A synthesized peptide derived from human AhR
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 96 kDa , observed, 105 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P35869 )

Gene NameAHR
Other Gene Names
AHR; Aryl Hydrocarbon Receptor; BHLHe76; Class E Basic Helix-Loop-Helix Protein 76; Ah Receptor; BHLHE76; Aromatic Hydrocarbon Receptor; AH-Receptor; RP85; AhR
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