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KD-Validated Anti-BTF3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1980
Application
WB, FC, ICC
Uniprot Id
P20290
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-BTF3 antibody (Cat#ABG1980). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BTF3 antibody (Cat#ABG1980, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-BTF3 antibody (Cat#ABG1980). BTF3 expression in wild-type (WT) and BTF3 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BTF3 antibody (Cat#ABG1980, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of BTF3 expression in HepG2 cells using anti-BTF3 antibody (Cat#ABG1980, 1:2,000). Green, isotype control; red, BTF3.
  • Immunocytochemical staining of HepG2 cells with anti-BTF3 antibody (Cat#ABG1980, 1:1,000). Nuclei were stained blue with DAPI; BTF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1980-100 3 days 100 µL $499.00
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ABG1980-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-BTF3 Rabbit Monoclonal Antibody
Accession (Primary)P20290
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6880
Immunogen
Recombinant protein of human BTF3
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 22 kDa, observed, 18,22 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P20290 )

Gene NameBTF3
Other Gene Names
BTF3; Basic Transcription Factor 3; BTF3a; BTF3b; NACB; Nascent-Polypeptide-Associated Complex Beta Polypeptide; Nascent Polypeptide-Associated Complex Subunit Beta; RNA Polymerase B Transcription Factor 3; Transcription Factor BTF3; NAC-Beta; BETA-NAC
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ADMEbio Team

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