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KD-Validated Anti-CD13 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1179
Application
WB, FC, ICC
Uniprot Id
P15144
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CD13 antibody (Cat#ABG1179). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD13 antibody (Cat#ABG1179, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CD13 antibody (Cat#ABG1179). CD13 expression in wild type (WT) and CD13 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD13 antibody (Cat#ABG1179, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CD13 expression in HT-1080 cells using CD13 antibody (Cat#ABG1179, 1:2,000). Green, isotype control; red, CD13.
  • Immunocytochemical staining of HT-1080 cells with CD13 antibody (Cat#ABG1179, 1:1,000). Nuclei were stained blue with DAPI; CD13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1179-100 3 days 100 µL $499.00
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ABG1179-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CD13 Rabbit Monoclonal Antibody
Accession (Primary)P15144
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3360
Immunogen
A synthesized peptide derived from human CD13
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 110 kDa, observed, 150 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P15144 )

Gene NameANPEP
Other Gene Names
ANPEP; Alanyl Aminopeptidase, Membrane; AP-N; HAPN; Microsomal Aminopeptidase; Aminopeptidase N; Aminopeptidase M; LAP1; P150; CD13; PEPN; Myeloid Plasma Membrane Glycoprotein CD13; Alanyl (Membrane) Aminopeptidase; Membrane Alanyl Aminopeptidase; EC 3.4.11.2; Gp150; GP150; AP-M; APN; Alanyl; Aminopeptidase; CD13 Antigen; EC 3.4.11
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ADMEbio Team

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