Western blotting analysis using anti-cAMP responsive element binding protein 1 antibody (Cat#ABG2098). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cAMP responsive element binding protein 1 antibody (Cat#ABG2098, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Western blotting analysis using anti-cAMP responsive element binding protein 1 responsive element binding protein 1 antibody (Cat#ABG2098). CAMP responsive element binding protein 1 responsive element binding protein 1 expression in wild-type (WT) and CREB1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cAMP responsive element binding protein 1 responsive element binding protein 1 antibody (Cat#ABG2098, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Flow cytometric analysis of cAMP responsive element binding protein 1 expression in C2C12 cells using anti-cAMP responsive element binding protein 1 antibody (Cat#ABG2098, 1:1,000). Green, isotype control; red, cAMP responsive element binding protein 1.

Immunocytochemical staining of C2C12 cells with anti-CAMP responsive element binding protein 1 antibody (Cat#ABG2098, 1:1,000). Nuclei were stained blue with DAPI; CAMP responsive element binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.