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KD-Validated Anti-Chromobox Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1303
Application
WB, FC, ICC
Uniprot Id
P45973
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-chromobox 5 antibody (Cat#ABG1303). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 5 antibody (Cat#ABG1303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-chromobox 5 antibody (Cat#ABG1303). Chromobox 5 expression in wild-type (WT) and chromobox 5 (CBX5) knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 5 antibody (Cat#ABG1303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#ABG1303, 1:2,000). Green, isotype control; red, Chromobox 5.
  • Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#ABG1303, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1303-100 3 days 100 µL $499.00
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ABG1303-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Chromobox Rabbit Monoclonal Antibody
Accession (Primary)P45973
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2865
Immunogen
A synthesized peptide derived from human HP1 alpha
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 22 kDa; observed, 22 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P45973 )

Gene NameCBX5
Other Gene Names
CBX5; Chromobox ; HP1-ALPHA; HP1; Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila); Heterochromatin Protein 1 Homolog Alpha; Chromobox Protein Homolog 5; HP1Hs-Alpha; Antigen P25; HP1alpha; HP1A; Chromobox Homolog 5 (Drosophila HP1 Alpha); Heterochromatin Protein 1-Alpha; HP1 Alpha Homolog (Drosophila); Epididymis Luminal Protein 25; Chromobox Homolog 5; HP1 Alpha Homolog; HP1Hs Alpha; HP1 Alpha; HEL25
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