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KD-Validated Anti-EIF2AK2 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1993
Application
WB, FC, ICC
Uniprot Id
P19525
Reactivity
Human
 
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  • Western blotting analysis using anti-EIF2AK2 antibody (Cat#ABG1993). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2AK2 antibody (Cat#ABG1993, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-EIF2AK2 antibody (Cat#ABG1993). EIF2AK2 expression in wild-type (WT) and EIF2AK2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF2AK2 antibody (Cat#ABG1993, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of EIF2AK2 expression in HepG2 cells using anti-EIF2AK2 antibody (Cat#ABG1993, 1:2,000). Green, isotype control; red, EIF2AK2.
  • Immunocytochemical staining of HepG2 cells with anti-EIF2AK2 antibody(Cat#ABG1993, 1:1,000). Nuclei were stained blue with DAPI; EIF2AK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1993-100 3 days 100 µL $499.00
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ABG1993-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-EIF2AK2 Mouse Monoclonal Antibody
Accession (Primary)P19525
Host SpeciesMouse
ClonalityMonoclonal
Clone Names24GB7125
Immunogen
Recombinant protein of human PKR
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 62 kDa, observed, 68 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P19525 )

Gene NameEIF2AK2
Other Gene Names
EIF2AK2; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; PKR; Protein Kinase R; PPP1R83; PRKR; Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent; Interferon-Induced, Double-Stranded RNA-Activated Protein Kinase; Protein Phosphatase 1, Regulatory Subunit 83; Tyrosine-Protein Kinase EIF2AK2; Protein Kinase RNA-Activated; Protein Kinase RNA-Regulated; P1/EIF-2A Protein Kinase; EIF-2A Protein Kinase 2; P68 Kinase; Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2; Interferon-Inducible RNA-Dependent Protein Kinase; Double Stranded RNA Activated Protein Kinase; Interferon-Inducible ElF2alpha Kinase; EC 2.7.11.1; EC 2.7.10.2; EIF2AK1; LEUDEN; DYT33
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