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KD-Validated Anti-IRAK3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1959
Application
WB, FC, ICC
Uniprot Id
Q9Y616
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-IRAK3 antibody (Cat#ABG1959). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IRAK3 antibody (Cat#ABG1959, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-IRAK3 antibody (Cat#ABG1959). IRAK3 expression in wild type (WT) and IRAK3 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-IRAK3 antibody (Cat#ABG1959, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of IRAK3 in HAP-1 cells using IRAK3 antibody (Cat#ABG1959, 1:2,000). Green, isotype control; red, IRAK3.
  • Immunocytochemical staining of HAP-1 cells with IRAK3 antibody (Cat#ABG1959, 1:1,000). Nuclei were stained blue with DAPI; IRAK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1959-100 3 days 100 µL $499.00
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ABG1959-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-IRAK3 Rabbit Monoclonal Antibody
Accession (Primary)Q9Y616
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB875
Immunogen
A synthesized peptide derived from human IRAK3
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 68 kDa , observed, 68 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9Y616 )

Gene NameIRAK3
Other Gene Names
Interleukin 1 Receptor Associated Kinase 3; Interleukin-1 Receptor-Associated Kinase 3; IRAK-M; Inactive IL-1 Receptor-Associated Kinase 3; IL-1 Receptor-Associated Kinase M; IRAK-3; ASRT5; IRAKM
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