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KD-Validated Anti-Inhibin subunit beta B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1062
Application
WB, FC, ICC
Uniprot Id
P09529
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#ABG1062). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#ABG1062, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#ABG1062). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#ABG1062, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#ABG1062, 1:2,000). Green, isotype control; red, inhibin subunit beta B.
  • Immunocytochemical staining of HT-1080 cells with anti-inhibin subunit beta B antibody (Cat#ABG1062, 1:1,000). Nuclei were stained blue with DAPI; Inhibin subunit beta B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1062-100 3 days 100 µL $499.00
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ABG1062-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Inhibin subunit beta B Rabbit Monoclonal Antibody
Accession (Primary)P09529
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6100
Immunogen
A synthesized peptide derived from human Inhibin beta B
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 45 kDa, observed, 52 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P09529 )

Gene NameINHBB
Other Gene Names
INHBB; Inhibin Subunit Beta B; Inhibin Beta B Chain; Activin Beta-B Chain; Inhibin, Beta B (Activin AB Beta Polypeptide); Activin AB Beta Polypeptide; Inhibin Beta B Subunit; Inhibin, Beta B; Inhibin, Beta-2
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ADMEbio Team

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