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KD-Validated Anti-LEFTY1 + LEFTY2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1068
Application
WB, ICC
Uniprot Id
O00292
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#ABG1068). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#ABG1068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#ABG1068). LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#ABG1068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-LEFTY1 + LEFTY2 antibody (Cat#ABG1068, 1:1,000). Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1068-100 3 days 100 µL $499.00
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ABG1068-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-LEFTY1 + LEFTY2 Rabbit Monoclonal Antibody
Accession (Primary)O00292
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6115
Immunogen
A synthesized peptide derived from human LEFTY1 + LEFTY2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 41 kDa, observed, 34 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O00292 )

Gene NameLEFTY2
Other Gene Names
LEFTY1; Left-Right Determination Factor 1; LEFTYB; LEFTB; Left-Right Determination Factor B; Protein Lefty-1; Protein Lefty-B; Left-Right Determination, Factor B; LEFTY2; Left-Right Determination Factor 2; LEFTYA; LEFTA; TGFB4; EBAF; Endometrial Bleeding-Associated Factor; Left-Right Determination Factor A; Transforming Growth Factor Beta-4; Protein Lefty-2; Protein Lefty-A; TGF-Beta-4; Endometrial Bleeding Associated Factor (Left-Right Determination, Factor A; Transforming Growth Factor Beta Superfamily); Transforming Growth Factor, Beta-4 (Endometrial Bleeding-Associated Factor; LEFTY A)
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