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KD Validated Anti-Phospho-p53 (S33) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1118
Application
WB, FC
Uniprot Id
P04637
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-p53 (S33) antibody (Cat#ABG1118). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p53 (S33) antibody (Cat#ABG1118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-p53 (S33) antibody (Cat#ABG1118). Phospho-p53 (S33) expression in wild type (WT) and TP53 knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-p53 (S33) antibody (Cat#ABG1118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Phospho-p53 (S33) expression in HeLa cells using anti-Phospho-p53 (S33) antibody (Cat# ABG1118, 1:2,000). Green, isotype control; red, Phospho-p53 (S33).
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1118-100 In Stock 100 µL $299.00
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ABG1118-20 In Stock 20 µL $89.00
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Product Overview

NameKD Validated Anti-Phospho-p53 (S33) Rabbit Monoclonal Antibody
Accession (Primary)P04637
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB600
Immunogen
A synthesized peptide derived from human Phospho-p53 (S33)
Calculated MW
Predicted, 44 kDa, observed, 53 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P04637 )

NameTP53
Other Names
TP53; Tumor Protein P53; P53; LFS1; Cellular Tumor Antigen P53; Phosphoprotein P53; Antigen NY-CO-13; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; Tumor Suppressor P53; Tumor Supressor P53; Tumor Protein 53; BMFS5; TRP53; BCC7
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