Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#ABG2304). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha smooth muscle Actin antibody (Cat#ABG2304, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#ABG2304). alpha smooth muscle Actin antibody expression in wild type (WT) and alpha smooth muscle Actin antibody shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-alpha smooth muscle Actin antibody (Cat#ABG2304, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of alpha smooth muscle Actin expression in HT-1080 cells using alpha smooth muscle Actin antibody (Cat#ABG2304, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.

Immunocytochemical staining of HT-1080 cells with alpha smooth muscle Actin antibody (Cat#ABG2304, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.