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KD-Validated Anti-c-Jun Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1128
Application
WB, FC, ICC
Uniprot Id
P05412
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-c-Jun antibody (Cat#ABG1128). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-c-Jun antibody (Cat#ABG1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-c-Jun antibody (Cat#ABG1128). C-Jun expression in wild-type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-c-Jun antibody (Cat#ABG1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of c-Jun expression in HT-1080 cells using c-Jun antibody (Cat#ABG1128, 1:2,000). Green, isotype control; red, c-Jun.
  • Immunocytochemical staining of HT-1080 cells with C-Jun antibody (Cat#ABG1128, 1:1,000). Nuclei were stained blue with DAPI; C-Jun was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1128-100 3 days 100 µL $499.00
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ABG1128-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-c-Jun Rabbit Monoclonal Antibody
Accession (Primary)P05412
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2435
Immunogen
A synthesized peptide derived from human c-Jun
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 36 kDa , observed, 39 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P05412 )

Gene NameJUN
Other Gene Names
JUN; Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; V-Jun Avian Sarcoma Virus 17 Oncogene Homolog; AP-1; Transcription Factor AP-1 Subunit Jun; Transcription Factor Jun; Proto-Oncogene C-Jun; Activator Protein 1; Jun Oncogene; C-Jun; AP1; P39; V-Jun Sarcoma Virus 17; Oncogene Homolog; Jun Activation Domain Binding Protein; Enhancer-Binding Protein AP1; Transcription Factor AP-1; Proto-Oncogene CJun; C-JUN; CJUN
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