Hello, Sign in
My Account
My Account
New customer? Start here
| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0308-2 | 7 days | 2 µg | $120.00 |
Select product before adding to cart
|
||
| GRF0308-10 | 7 days | 10 µg | $290.00 | |||
| GRF0308-1 | 7 days | 1 mg | $5,616.00 |
Product Overview | |
| Name | PDGF BB Human |
|---|---|
| Description | |
| Platelet-Derived Growth Factor BB Human Recombinant | |
| Accession (Primary) | P01127 |
| Synonyms | |
| Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin. | |
| Introduction | |
| PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| The protein was lyophilized from 10mM NaCitrate pH-3.0. | |
| Stability | |
| Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT | |
| Biological Activity | |
| The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Background | |
| Platelet-Derived Growth Factor BB (PDGF-BB) is one of the isoforms of the PDGF family that plays a significant role in embryonic development, cell proliferation, cell migration, and angiogenesis. It's also relevant in the context of certain pathologies like cancer or healing processes. Here's what a research paper on Mouse Recombinant PDGF-BB might involve: Basic Biology: It might discuss the basic biology and function of PDGF-BB in the mouse model. This could include its role in various cellular processes like cell growth, division, and survival, as well as in the context of tissue repair or angiogenesis. Recombinant PDGF-BB Production: The paper might describe methods for producing recombinant PDGF-BB in laboratory conditions. It might go into detail on the expression system used (e.g., bacterial, yeast, or mammalian cells), the purification process, and how the functionality of the produced PDGF-BB was confirmed. Applications: It could also detail the experimental applications of recombinant PDGF-BB in the mouse model. For instance, it could be used to study wound healing processes, tissue regeneration, or the development and progression of certain diseases. Comparative Analysis: The research might include a comparison of the recombinant PDGF-BB with its natural counterpart in terms of functionality, structure, or other properties. Innovation: If the paper is presenting new research, it might discuss an innovative use of PDGF-BB, a novel production method, or new findings about its function. | |
| References | |
| 1. Title: Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip Publications: Lab on a Chip (2015) Link: PDGF BB Mouse prospec publication 2. Title: The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs Publication: Cold Spring Harbor Protocols 2010.4 (2010): pdb-prot5416. Link: PDGF B-chain prospec publication | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. | |
| Precautions | |
| PDGF BB Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P01127 ) | |
Background |
PDGF BB HUMAN: Overview of Its Production, Properties, and Clinical Significance PDGF BB HUMAN, standing for Platelet-Derived Growth Factor BB, is a powerful protein in the field of medical research, particularly in cell growth and healing. This growth factor plays a crucial role in the development and repair of tissues by stimulating cells primarily of mesenchymal origin. Characteristics and Production PDGF is produced as a recombinant protein in E. coli. It presents as a homodimer consisting of two identical polypeptide chains, each containing 109 amino acids, culminating in a total molecular mass of 24.3 kDa. The production process ensures a high-purity product, which is essential for reliable scientific results. Physical Properties and Formulation This growth factor appears as a white, sterile, lyophilized powder. It is formulated in a buffered solution (PBS, pH 7.4) and then filtered to ensure sterility and purity, essential for laboratory use. The formulation process is designed to maintain the stability and activity of the protein under various research conditions. Solubility and Storage Instructions PDGF BB is recommended to be reconstituted in sterile water to achieve a concentration of no less than 100 µg/ml. This solution can then be diluted further to meet experimental needs. Once reconstituted, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage. Avoiding freeze-thaw cycles is crucial to preserve its biological activities. Stability and Purity The lyophilized form of PDGF BB remains stable at room temperature for up to three weeks but requires desiccation for longer storage. Moreover, the purity of this growth factor exceeds 95%, as confirmed by rigorous testing, including RP-HPLC and SDS-PAGE, ensuring that researchers receive a highly effective product. Research Applications PDGF BB HUMAN is widely used in laboratory research to explore various biological processes, including wound healing, angiogenesis, and the development of certain types of cancers. It is also instrumental in studying the cellular mechanisms underlying tissue repair and regeneration. Biological Activity The effectiveness of PDGF BB is measured by its ability to stimulate the proliferation of Balb/c 3T3 cells, with an effective dose (ED50) ranging from 1.0 to 3.0 ng/ml. This high level of activity underscores its utility in promoting cell growth, making it an invaluable tool in tissue engineering and regenerative medicine. Usage Guidelines It is important to note that PDGF BB HUMAN is intended solely for laboratory research and is not suitable for drug, food, or cosmetic applications. Researchers must handle this growth factor under controlled conditions to ensure safety and efficacy. Essentially, PDGF BB is a pivotal component in the toolkit of biomedical researchers, offering profound insights into cellular processes and potential therapeutic approaches. Also, its well-defined properties and controlled production make it a staple in studies focused on cell growth and tissue repair. |
References |
1. Title :Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway. Publication :Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol. 115 no. 1 51-60 . Link : PDGF BB prospec publication 2. Title : attenuates skeletal muscle dystrophy in mdx mice. Publication :Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548. Link : Platelet-Derived Growth Factor BB prospec publication 3. Title : Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing. Publication : Cell Transplantation, Vol. 19, pp. 1635–1644, 2010 0963-6897/10 $90.00 + .00 Printed in the USA. All rights reserved. DOI: 10.3727/096368910X516637 Copyright ? 2010 Cognizant Comm. Corp. Link : http://docserver.ingentaconnect.com/deliver/connect/cog/09636897/v19n12/s11.pdf?expires=1370351771&id=74450861&titleid=5476&accname=Guest+User&checksum= 14BACCDE2ACF33 |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
ADMEbio welcomes feedback from our customers.
Thank you,
ADMEbio Team
