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Phospho-ER alpha (Ser118) Rabbit mAb

Catalog #: ABZ4594
Application: WB, IF, ICC, IHC-P
Uniprot Id: P03372
Reactivity: Human
Isotype: IgG

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Western blot analysis of phospho-Estrogen Receptor alpha (Ser118) in MCF7 lysates using phospho-Estrogen Receptor alpha (Ser118) antibody.
Western blot analysis of Phospho-ER alpha (S118) in (1) MCF-7 lysates; (2) MCF-7 lysates treated with bEstradiol and EGF using Phospho-Estrogen Receptor alpha (Ser118) antibody.
Immunohistochemistry analysis of paraffin-embedded Human kidney using Phospho-ER alpha (S118) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence analysis of Phospho-Estrogen Receptor alpha (Ser118) in MCF-7 treated with EGF using Phospho-ER alpha (S118) antibody.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	Phospho-ER alpha (Ser118) Rabbit mAb
Accession (Primary)	P03372
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human
Calculated MW
Calculated MW: 66 kDa; Observed MW: 66 kDa
Buffer System
Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; ICC/IF-1:100-1:200; IHC-1:50-1:100
Purification
Affinity Chromatography
Conjugation
Unconjugated
Modification
Phosphorylated
Form
Liquid
Background
ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.
Target Information: ( P03372 )
Name	ESR1
Gene ID	2099
Other Names
Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1; ESR; NR3A1

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