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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP0054-20 | 7 days | 20 µg | $160.00 |
Select product before adding to cart
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| RCP0054-100 | 7 days | 100 µg | $500.00 | |||
| RCP0054-1 | 7 days | 1 mg | $2,880.00 |
Product Overview | |
| Name | Ang K1-3 Human |
|---|---|
| Description | |
| Angiostatin Kringles 1-3 Human Recombinant | |
| Introduction | |
| There are several proteolytic fragments or specific domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein kringles 1-4 and kringles 1-5, Endostatin, Restin, PEX, the N-terminal fragment of prolactin, and the Nterminally truncated platelet factor. Angiostatin is a proteolytic protein fragment of plasminogen that is comprised of the first 4 kringle regions. Angiostatin k1-4 prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Angiostatin Kringles 1-3 segment has a larger inhibitory activity than the Angiostatin kringles 1-4 fragment. The protease-activated angiostatin kringles 1-5 is the most potent plasminogen fragment with over 50 times larger endothelial cell specific inhibitory activity. Angiostatin kringles 1-5 systemic administration inhibits growth of fibrosarcoma and significantly reduces neovascularization. Angiostatin is an angiogenesis inhibitor in mouse serum and urine. Angiostatin is a 38 kDa protein fragment of the plasminogen composed of the 1st 4 kringle domains of plasminogen. Angiostatin K1-4 is also named plasminogen kringles 1-4 and PK1-4. Angiostatin protein is manufactured by the protelytic cleavage of plasminogen by a serine protease from several prostate carcinoma cell lines. The manufacturing of angiostatin by pancreatic cancer cells can be inhibited by TGF-beta 1 along with plasminogen activator inhibitor type-1 (PAI1). | |
| Source | |
| Human Fluid. | |
| Physical Appearance | |
| Sterile Filtered lyophilized powder. | |
| Formulation | |
| Lyophilized from a (1mg/ml) solution in containing 20mM Hepes buffer pH-8.2 & 20mM NaCl. | |
| Stability | |
| Lyophilized Angiostatin Kringles 1-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Angiostatin Kringles1-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by SDS-PAGE. | |
| Biological Activity | |
| Human Angiostatin Kringles 1-4 significantly inhibits basic-FGF induced endothelial cell proliferation and migration at concentration ranging from 300nM-1.0 uM. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Angiostatin K1-4 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Precautions | |
| Ang K1-3 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
References |
Title: Regenerative protein thymosin b-4 is a novel regulator of purinergic signaling. Publication: Published online before printNovember 24, 2010, doi: 10.1096/fj.10-169417 March 2011 The FASEB Journal vol. 25no. 3907-915. Link: http://www.fasebj.org/content/25/3/907.full.pdf+html |
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