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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP0418-2 | 7 days | 2 µg | $120.00 |
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| RCP0418-10 | 7 days | 10 µg | $290.00 | |||
| RCP0418-100 | 7 days | 100 µg | $1,920.00 |
Product Overview | |
| Name | CHCHD7 Human |
|---|---|
| Description | |
| Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 7 Human Recombinant | |
| Accession (Primary) | Q9BUK0 |
| Synonyms | |
| Chromodomain Helicase DNA binding protein 4, Mi-2b, Mi2-BETA, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, EC 3.6.4.12, EC 3.6.1. | |
| Introduction | |
| CHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults. | |
| Source | |
| Sf9 insect cells. | |
| Formulation | |
| CHD4 is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Greater than 95% as determined by SDS-PAGE. | |
| Applications | |
| Western blot with myositis sera ormonoclonal anti-hexa-His-tag antibody. | |
| Immunological functions | |
| 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. | |
| Coating concentration | |
| 0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. | |
| Precautions | |
| CHCHD7 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q9BUK0 ) | |
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ADMEbio Team
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