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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP0801-250 | 7 days | 250I µ | $2,640.00 |
Select product before adding to cart
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| RCP0801-500 | 7 days | 500I µ | $4,840.00 | |||
| RCP0801-1000 | 7 days | 1000I µ | $6,600.00 |
Product Overview | |
| Name | F8 Protein |
|---|---|
| Description | |
| Coagulation Factor-VIII Human Recombinant | |
| Accession (Primary) | P00451 |
| Immunogen | |
| Human Factor-IX. | |
| Introduction | |
| Factor IX is a glycoprotein, which is synthesized in the liver. The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues, which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180). Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa, which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the “intrinsic factor Xase complex” (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa. | |
| Formulation | |
| 1mg/ml in PBS (after reconstitution). | |
| Solubility | |
| Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. | |
| Applications | |
| Direct ELISA, Western Blot, imunohistochemistry. For W.B. use 1 µg/ml of antibody. | |
| Type | |
| Mouse Antibody Monoclonal. | |
| Clone | |
| NYRhFIX. | |
| Ig Subclass | |
| Mouse IgG1. | |
| Purification Method | |
| Protein A. | |
| Storage Procedures | |
| In lyophilized form, for long periods, store at 4 C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 C. | |
| Shipping Conditions | |
| Antibody is shipped lyophilized at ambient temperature. | |
| Titer | |
| By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). | |
| Precautions | |
| F8 Protein is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P00451 ) | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
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Thank you,
ADMEbio Team
