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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP1527-5 | 7 days | 5 µg | $120.00 |
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| RCP1527-20 | 7 days | 20 µg | $290.00 | |||
| RCP1527-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | NSMCE1 Human |
|---|---|
| Description | |
| Non-SMC Element 1 Human Recombinant | |
| Accession (Primary) | Q8WV22 |
| Synonyms | |
| Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3. | |
| Introduction | |
| NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| Lyophilized from 0.02% TFA. | |
| Stability | |
| Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT. | |
| Biological Activity | |
| The ED 50 as determined by the dose-dependent proliferation of C6 cells and is 3.6-5.4?g/ml. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| 1. Title :Generation of Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant[1]^†[2]^‡. Publication :Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press. Link :http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full Links: 1. NT 3 prospec publication 2. NT 3 Human prospec publication 2. Title :Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action. Publication : Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008. Molecular Therapy (2008) 16 11, 1873–1882 doi:10.1038/mt.2008.189 Link :http://www.nature.com/mt/journal/v16/n11/full/mt2008189a.html 3. Title: TrkA Receptor “Hot Spots” for Binding of NT-3 as a Heterologous Ligand Publications: Journal of Biological Chemistry 282.23 (2007): 16754-16763. Link: Neurotrophin-3 prospec publication 4. Title: Orphan nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanism Publication: FEBS letters 583.9 (2009): 1505-1510 Link NGF-2 prospec publication | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard. | |
| Precautions | |
| NSMCE1 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q8WV22 ) | |
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