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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP1597-5 | 7 days | 5 µg | $120.00 |
Select product before adding to cart
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| RCP1597-25 | 7 days | 25 µg | $290.00 | |||
| RCP1597-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | PCNA Human |
|---|---|
| Description | |
| Proliferating Cell Nuclear Antigen Human Recombinant | |
| Accession (Primary) | P12004 |
| Synonyms | |
| Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367. | |
| Introduction | |
| Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis. Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE. | |
| Source | |
| Sf9 insect cells. | |
| Physical Appearance | |
| Sterile Filtered colorless solution. | |
| Formulation | |
| Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Greater than 95% as determined by SDS-PAGE. | |
| Applications | |
| Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. | |
| Immunological functions | |
| 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera. | |
| Coating concentration | |
| 0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. | |
| Precautions | |
| PCNA Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P12004 ) | |
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Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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ADMEbio Team
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ADMEbio Team
