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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP1636-5 | 7 days | 5 µg | $120.00 |
Select product before adding to cart
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| RCP1636-25 | 7 days | 25 µg | $290.00 | |||
| RCP1636-1 | 7 days | 1 mg | $3,600.00 |
Product Overview | |
| Name | PFN4 Human |
|---|---|
| Description | |
| Profilin-4 Human Recombinant | |
| Accession (Primary) | Q8NHR9 |
| Synonyms | |
| DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase. | |
| Introduction | |
| Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus, where it functions in vivoto replicate the organism's DNA. In vitro, Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction, where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and 'proofreading' properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity, meaning that it works its way along the DNA from the 5' endto the 3' endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile liquid formulation. | |
| Formulation | |
| 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol. | |
| Stability | |
| Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C. Please prevent freeze-thaw cycles. | |
| Applications | |
| 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3’ overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75°C. 7. 95% active after 1-hour incubation at 98°C. | |
| Unit Definition | |
| 1U of enzyme catalyzes the incorporation of 10nmol of dNTP into acid-insoluble product in 30 minutes at 75°C. | |
| 10X Buffer with MgSO4 | |
| 200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH 4 ) 2 SO 4 , 100mM KCl, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO 4 . | |
| PCR Protocol | |
| Add the following components to amplify 1kb DNA template: 0.2 µl Pfu-DNA Polymerase. 4 µl 2.5mM dNTPs. 5 µl 10x buffer with MgSO 4 . 1 µl Primers mix (10 µM each). 1 µl Template. 38 µl ddH 2 O. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94°C/4 min. Denaturation: 30 cycles at 94°C/30 sec. Annealing: 30 cycles at 55°C /30 sec. Extension: 30 cycles at 72°C /90 sec. Final: 1 cycle at 72°C /5 min. | |
| Precautions | |
| PFN4 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q8NHR9 ) | |
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ADMEbio Team
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