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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP1697-2 | 7 days | 2 µg | $120.00 |
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| RCP1697-10 | 7 days | 10 µg | $290.00 | |||
| RCP1697-1 | 7 days | 1 mg | $8,320.00 |
Product Overview | |
| Name | PPP1R11 Human |
|---|---|
| Description | |
| Protein Phosphatase 1, Regulatory Subunit 11 Human Recombinant | |
| Accession (Primary) | O60927 |
| Immunogen | |
| Anti-human PPP1R14A mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPP1R14A amino acids 1-147 purified from E. coli. | |
| Synonyms | |
| Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, CPI17, CPI-17, PPP1INL, PPP1R14A. | |
| Introduction | |
| PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. Inhibition of PPP1R14A results to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Human pregnancy is characterized by the increases of PKN1 expression and CPI-17 phosphorylation in the myometrium. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. PPP1R14A siRNA decreased the level of merlin phosphorylation and consequently Ras and ERK activity in human tumor cell lines. PKC/CPI-17 medi | |
| Formulation | |
| 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | |
| Applications | |
| PPP1R14A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500. | |
| Type | |
| Mouse Anti Human Monoclonal. | |
| Clone | |
| P4H10AT. | |
| Ig Subclass | |
| Mouse IgG2a heavy chain and κ light chain. | |
| Purification Method | |
| PPP1R14A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | |
| Storage Procedures | |
| For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | |
| Stability / Shelf Life | |
| 12 months at -20°C. 1 month at 4°C. | |
| Precautions | |
| PPP1R11 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( O60927 ) | |
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ADMEbio Team
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ADMEbio Team
