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Soybean P34 GST

Soybean P34 Protein Recombinant, GST Tag

 
Catalog #
RCP2203
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
RCP2203-100 7 days 100 µg $330.00
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RCP2203-500 7 days 500 µg $1,200.00
RCP2203-1000 7 days 1000 µg $2,160.00
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Product Overview

NameSoybean P34 GST
Description
Soybean P34 Protein Recombinant, GST Tag
Synonyms
Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b.
Introduction
SP100 protein is Homodimer which takes part in the control of gene expression. SP100 interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif and also interacts with Epstein-Barr virus EBNA-LP. The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa.
Source
Sf9 insect cells.
Formulation
Sp100 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Applications
Western blot with PBC sera or monoclonal anti-hexa-His-tag antibody.
Immunological functions
1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test.
Coating concentration
0.5-0.9 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Precautions
Soybean P34 GST is for research use only and not for use in diagnostic or therapeutic procedures.
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