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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP2241-2 | 7 days | 2 µg | $120.00 |
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| RCP2241-10 | 7 days | 10 µg | $290.00 | |||
| RCP2241-1 | 7 days | 1 mg | $8,320.00 |
Product Overview | |
| Name | SRP19 Human |
|---|---|
| Description | |
| Signal Recognition Particle 19kDa Human Recombinant | |
| Accession (Primary) | P09132 |
| Synonyms | |
| Signal Recognition Particle 54 kDa protein | |
| Introduction | |
| SRP54 is a 54-kDa subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex which leads the translocation of newly synthesized secretory proteins from the polysome to the endoplasmic reticulum. SRP is composed of six polypeptides and a tRNA-like molecule known as 7SL RNA. SRP54 is a GTP-binding protein that directly binds the signal sequences of nascent secretory and membrane proteins. Anti-SRP autoantibodies take place in patients with an autoimmune chronic muscle inflammation called polymyositis. These auto-antibodies predominantly recognize the SRP54 subunit and in addition are able to immunoprecipitate several of the SRP subunits and the RNA component. Nearly 5% of myositis patients are positive for anti-SRP autoantibodies, increasing to 18% in the subgroup of Jo-1 autoantibody-negative patients. The classic 'anti-SRP syndrome' is a severe form of polymyositis in which the myositic inflammation is acute and aggressive onset, with common myalgias and cardiac involvement. Normally there is a poor reaction to therapy with a 5-year survival rate of about 25%. | |
| Source | |
| Sf9 insect cells. | |
| Formulation | |
| SRP54 is supplied in 16mM HEPES buffer pH-8.0, 320mM NaCl, 5mM DTT, 1mM EDTA, 0.16mM GDP and 20% glycerol. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Greater than 80% as determined by SDS-PAGE. | |
| Applications | |
| Western blot with myositis sera or monoclonal anti-hexa-His-tag antibody. | |
| Immunological functions | |
| 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. | |
| Coating concentration | |
| 0.5-0.9 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. | |
| Precautions | |
| SRP19 Human is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P09132 ) | |
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ADMEbio Team
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