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Streptavidin (37-159), His

Streptavidin (37-159 a.a) Recombinant, His Tag

 
Catalog #
RCP2274
Uniprot Id
P22629
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
RCP2274-5 7 days 5 µg $120.00
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RCP2274-20 7 days 20 µg $290.00
RCP2274-1 7 days 1 mg $4,320.00
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Product Overview

NameStreptavidin (37-159), His
Description
Streptavidin (37-159 a.a) Recombinant, His Tag
Accession (Primary)P22629
Immunogen
Anti Streptavidin mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant Streptavidin amino acids 25-183 purified from E. coli.
Introduction
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Physical Appearance
Sterile Filtered clear solution.
Formulation
1mg/ml containing PBS, pH-7.4, 0.02% Sodium Azide and 10% glycerol.
Applications
Streptavidin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Type
Mouse Antibody Monoclonal.
Clone
P1C2AT.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Purification Method
Streptavidin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.
Precautions
Streptavidin (37-159), His is for research use only and not for use in diagnostic or therapeutic procedures.

Target Information: ( P22629 )

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