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Streptavidin Protein

Streptavidin

 
Catalog #
RCP2275
Uniprot Id
P22629
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
RCP2275-2 7 days 2 mg $200.00
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RCP2275-10 7 days 10 mg $460.00
RCP2275-100 7 days 100 mg $2,976.00
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Product Overview

NameStreptavidin Protein
Description
Streptavidin
Accession (Primary)P22629
Introduction
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The Streptavidin protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Amino acid sequence
MVHHHHHH DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ.
References
Title :Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous interface in a plug based microfluidic system: characterization and testing with membrane protein crystallization. Publication :Department of Chemistry, The University of Chicago,929 East 57th Street, Chicago, Illinois 60637 E-mail: r-ismagilov@uchicago.edu Link : http://ismagilovlab.caltech.edu/publications/ Ismagilov_JACS_2009_Mobile_Functional_SAMs_JEK_Supp_Info.pdf
Precautions
Streptavidin Protein is for research use only and not for use in diagnostic or therapeutic procedures.

Target Information: ( P22629 )

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