| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP2276-5 | 7 days | 5 µg | $120.00 |
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| RCP2276-20 | 7 days | 20 µg | $290.00 | |||
| RCP2276-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | Streptavidin, His |
|---|---|
| Description | |
| Streptavidin Recombinant, His Tag | |
| Accession (Primary) | P22629 |
| Introduction | |
| Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Liquid formulation. | |
| Formulation | |
| The sterile solution contains 10mM K 2 HPO 4 -KH 2 PO 4 , pH 7.3. | |
| Stability | |
| Streptavidin-NC although stable at 4°C for 3 weeks, should be stored below -18°C. Please prevent freeze thaw cycles. | |
| Purity | |
| Greater than 93.0% as determined by SDS-PAGE. | |
| Precautions | |
| Streptavidin, His is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P22629 ) | |
References |
Title :Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous interface in a plug based microfluidic system: characterization and testing with membrane protein crystallization. Publication :Department of Chemistry, The University of Chicago,929 East 57th Street, Chicago, Illinois 60637 E-mail: r-ismagilov@uchicago.edu Link : http://ismagilovlab.caltech.edu/publications/ Ismagilov_JACS_2009_Mobile_Functional_SAMs_JEK_Supp_Info.pdf |
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