Hello, Sign in
My Account
My Account
New customer? Start here
| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP2321-10 | 7 days | 10 µg | $120.00 |
Select product before adding to cart
|
||
| RCP2321-50 | 7 days | 50 µg | $290.00 | |||
| RCP2321-1 | 7 days | 1 mg | $3,840.00 |
Product Overview | |
| Name | TANK Human (1-119) |
|---|---|
| Description | |
| TRAF Family Member-Associated NFKB Activator Human Recombinant (1-119 a.a.) | |
| Accession (Primary) | Q92844 |
| Synonyms | |
| Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA. | |
| Source | |
| Recombinant E.coli contains Thermus aquaticus polymerase gene. | |
| Formulation | |
| 5U/ µl. | |
| Stability | |
| Stable for 5 days at 10°C, for longer period of time store at -20°C. | |
| Unit Definition | |
| One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. | |
| Note | |
| All reagents, including Taq Plus, should be mixed immediately before use. | |
| Storage Buffer | |
| 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol. | |
| Reaction Conditions | |
| DNA synthesis is performed in 100?l of mixture containing 20-200 µm dNTPs, 0.3-1 µm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. | |
| 10X Reaction Buffer | |
| 200 mM TrisHCI (pH 8.8) 100 mM KCI 100 mM (NH4)2SO4 20 mM Mg SO4 1% Triton X-100 1 mg/ml bovine serum albumin (BSA). | |
| Optimization of DNA Synthesis | |
| It is important to ad the reaction components in the following order1. H2O. 2. 10 x reaction buffer. 3. dNTPs. 4. DNA template and primers. 5. Taq Plus. | |
| Precautions | |
| TANK Human (1-119) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q92844 ) | |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
ADMEbio welcomes feedback from our customers.
Thank you,
ADMEbio Team
