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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP2455-1 | 7 days | 1 mg | $120.00 |
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| RCP2455-2.5 | 7 days | 2.5 mg | $290.00 | |||
| RCP2455-50 | 7 days | 50 mg | $1,440.00 |
Product Overview | |
| Name | Trypsin Porcine |
|---|---|
| Description | |
| Trypsin Porcine Recombinant | |
| Introduction | |
| Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl- L-arginine methyl ester (TAME), tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide (BAPNA), L-lysyl-p-nitroanilide, and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone), PMSF (phenylmethanesulfonyl fluoride), benzamidine, soybean trypsin inhibitor, and ovomucoid. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered lyophilized powder. | |
| Formulation | |
| The protein was lyophilized without any additives. | |
| Stability | |
| Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 90% as determined by SDS-PAGE. | |
| Biological Activity | |
| 2,800 units/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Human Trypsin in sterile 1mM HCl or 50mM HAC not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Applications | |
| Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w). | |
| Unit Definition | |
| One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path). | |
| Precautions | |
| Trypsin Porcine is for research use only and not for use in diagnostic or therapeutic procedures. | |
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Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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ADMEbio Team
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ADMEbio Team
