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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| RCP2515-2 | 7 days | 2 µg | $120.00 |
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| RCP2515-10 | 7 days | 10 µg | $290.00 | |||
| RCP2515-1 | 7 days | 1 mg | $8,320.00 |
Product Overview | |
| Name | UNC5B Rat |
|---|---|
| Description | |
| UNC-5 Netrin Receptor B Rat Recombinant | |
| Accession (Primary) | O08722 |
| Synonyms | |
| Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG. | |
| Source | |
| Escherichia Coli strain that carries the UNG gene from E.coli. | |
| Physical Appearance | |
| Sterile Filtered colorless solution. | |
| Formulation | |
| UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol. | |
| Stability | |
| Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles. | |
| Specific Activity | |
| The Specific Activity was found to be 5U/ µl. | |
| Unit Definition | |
| 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. | |
| Inactivation | |
| Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C. | |
| Reaction Conditions | |
| 1X UNG Reaction Buffer, incubate at 37°C. UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. | |
| 10X UNG Reaction Buffer | |
| 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA. | |
| Precautions | |
| UNC5B Rat is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( O08722 ) | |
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ADMEbio Team
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ADMEbio Team
