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Dengue NS1

Dengue Virus NS1 Recombinant

 
Catalog #
VAG0122
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
VAG0122-100 7 days 100 µg $330.00
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VAG0122-500 7 days 500 µg $1,320.00
VAG0122-1000 7 days 1000 µg $2,160.00
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Product Overview

NameDengue NS1
Description
Dengue Virus NS1 Recombinant
Physical Appearance
2 vials of sterile Filtered clear colorless solution.
Formulation
*Dengue NS1 Antibody solution for gold conjugation (3.6mg/ml) in PBS and Sodium nitrate. *Dengue NS1 Antibody solution for membrane coating (2.2mg/ml) in PBS and Sodium nitrate.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
95% pure (12% SDS-PAGE and Coomassie staining).
Applications
Lateral flow immunoassay.
Purification Method
Purified monoclonal IgG by protein A chromatography.
Assay Conditions
1. 1-2 days fresh prepared gold particle with maximal homogeneity in gold particle size, λ 525-529, OD 1.2-1.4. 2. Conjugation at pH 8.5, conjugation time should be 6-8 hours. 3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0- 8.2, add casein to the final 0.3-0.4%. 4. Treat sample pad with 50mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH8.0.
Precautions
Dengue NS1 is for research use only and not for use in diagnostic or therapeutic procedures.
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