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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| VAG0396-100 | 7 days | 100 µg | $440.00 |
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| VAG0396-0.5 | 7 days | 0.5 mg | $1,760.00 | |||
| VAG0396-1 | 7 days | 1 mg | $2,464.00 |
Product Overview | |
| Name | M.Pneumoniae P30 |
|---|---|
| Description | |
| Mycoplasma Pneumoniae P30 Recombinant | |
| Accession (Primary) | P75330 |
| Introduction | |
| M2 autoantigen is a key mark of antimitochondrial autoantibodies (AMA), a typical serological feature in patients suffering from primary biliary cirrhosis (PBC) which is a serious autoimmune liver disease accompanied by damage to intrahepatic bile ducts. Molecular definition of the M2 antigen has displayed it as no less than 3 separate target proteins. The M2 role is like the so-called E2 subunits (or dihydrolipoamide transferases) of different mitochondrial dehydrogenase complexes: *pyruvate dehydrogenase complex. *branched chain 2-oxo-acid dehydrogenase complex. *2-oxoglutarate dehydrogenase complex. Biochemically, these complexes catalyze the oxidative decarboxylation of various alpha-keto-acid substrates and systematically engage with a prosthetic lipoamide group; they are situated in the mitochondrial matrix in association with the inner membrane. The most well-known reactivity of AMA positive PBC sera is against PDC-E2. Some patients have AMA which reacts with PDC-E2 alone (95%), but most patients also show reactivity against OGDC-E2 (39-88%) and/or BCOADC-E2 (53-55%). Actually, patients can be found with reactivity only against OGDC-E2 and/or BCOADC-E2 and no PDC-E2 autoantibodies. These patients will be overlooked in assays based on natural source-derived, predominantly PDC-E2-containing M2 antigen preparations. | |
| Source | |
| Sf9 insect cells. | |
| Formulation | |
| M2 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Greater than 75% as determined by SDS-PAGE. | |
| Applications | |
| Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody. | |
| Immunological functions | |
| 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. | |
| Coating concentration | |
| 0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. | |
| Precautions | |
| M.Pneumoniae P30 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P75330 ) | |
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ADMEbio Team
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ADMEbio Team
