| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| VAG0513-100 | 7 days | 100 µg | $300.00 |
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| VAG0513-500 | 7 days | 500 µg | $1,200.00 | |||
| VAG0513-1000 | 7 days | 1000 µg | $1,920.00 |
Product Overview | |
| Name | Dengue p18 |
|---|---|
| Description | |
| Dengue p18 Recombinant | |
| Introduction | |
| Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus. | |
| Source | |
| Escherichia Coli. | |
| Formulation | |
| Phosphate buffered saline, pH 7.4 and 0.02% sodium nitrate. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Protein is >95% pure as determined by 12% PAGE (coomassie staining). | |
| Preparation protocol | |
| Protocol to prepare dengue IgG/IgM rapid test: 1. Prepare 532 to 534nm colloid gold, conjugation within 24 hours after colloid gold preparation; 2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 16 to 18 ug/ml gold and 7.2; 3. Shanking conjugation for 6-8 hours, then centrifuge the conjugation at 10,000 rpm at 18oC for 20 min; 4. Remove the supernatnant as more as possible, add thesupplied gold suspension buffer; 5. Add thesupplied blocking reagentto the final concentration to 0.4%; 6. Blocking for 1 hours , then add sucrose to the final concentration to 20%; 7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment); 8. After drying, assemble gold pad, thesupplied sample padand absorbent pad over backing card; 9. Add 10ul serum/plasma and add two drops running buffer for assay. | |
| Precautions | |
| Dengue p18 is for research use only and not for use in diagnostic or therapeutic procedures. | |
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Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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ADMEbio Team
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ADMEbio Team