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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List |
|---|
Product Overview | |
| Name | HIV-1 gp120 CM |
|---|---|
| Description | |
| HIV-1 gp120 CM Envelope Recombinant | |
| Accession (Primary) | L03703 |
| Introduction | |
| Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells. | |
| Source | |
| Baculovirus Insect Cells. | |
| Physical Appearance | |
| Sterile filtered colorless clear solution. | |
| Formulation | |
| The protein solution contains 10mM Tris-Cl pH-7.6, 150mM NaCl and 0.01% Triton N-101. | |
| Stability | |
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. | |
| Purity | |
| Greater than 90.0% as determined by HPLC analysis and SDS-PAGE. | |
| Amino acid sequence | |
| IPGEKLWVTV YYGVPVWKEA TTTLFCASDA KAYDTEVHNV ATHACVPTDP NPQEVVLVNV TENFNMWKND MVEQMHEDII SLWDQSLKPC VKLTPLCVSL KCTDLKNDTN TNSSSGRMIM EKGEIKNCSF NISTSIRDKV QKEYAFFYKL DIVPIDNTSY RLISCNTSVI TQACPKVSFE PIPIHYCAPA GFAILKCNNK TFNGTGPCTN VSTVQCTHGI RPVVSTQLLL NGSLAEEDVV IRSANFTDNA KTIIVQLNTS VEINCTRPNN NTRKSIRIQR GPGRAFVTIG KIGNMRQAHC NISRAKWNAT LKQIASKLRE QFGNNKTIIF KQSSGGDPEI VTHSFNCGGE FFYCNSTQLF NSTWFNSTWS TEGSNNTEGS DTITLPCRIK QFINMWQEVG KAMYAPPISG QIRCSSNITG LLLTRDGGNN NNGSEIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR. | |
| Applications | |
| HIV-1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. | |
| Specificity | |
| Immunoreactive with sera from HIV infected individuals. Western blots: 0.1-1.0 µg/strip. | |
| Precautions | |
| HIV-1 gp120 CM is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( L03703 ) | |
References |
Title :Slit2/Robo4 Signaling Modulates HIV-1 gp120-Induced Lymphatic Hyperpermeability. Publication :Zhang X, Yu J, Kuzontkoski PM, Zhu W, Li DY, et al. (2012) Slit2/Robo4 Signaling Modulates HIV-1 gp120-Induced Lymphatic Hyperpermeability. PLoS Pathog 8(1): e1002461. doi:10.1371/journal.ppat.1002461 Link : http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002461 |
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