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KD-Validated Anti-NSDHL Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1609
Application
WB, FC, ICC
Uniprot Id
Q15738
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-NSDHL antibody (Cat#ABG1609). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NSDHL antibody (Cat#ABG1609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-NSDHL antibody (Cat#ABG1609). NSDHL expression in wild type (WT) and NSDHL shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NSDHL antibody (Cat#ABG1609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of NSDHL expression in HeLa cells using NSDHL antibody (Cat#ABG1609, 1:2,000). Green, isotype control; red, NSDHL.
  • Immunocytochemical staining of HeLa cells with anti-NSDHL antibody (Cat#ABG1609, 1:1,000). Nuclei were stained blue with DAPI; NSDHL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1609-100 3 days 100 µL $499.00
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ABG1609-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NSDHL Rabbit Monoclonal Antibody
Accession (Primary)Q15738
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB485
Immunogen
A synthesized peptide derived from human NSDHL
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 42 kDa , observed , 38 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( Q15738 )

Gene NameNSDHL
Other Gene Names
NAD(P) Dependent Steroid Dehydrogenase-Like; SDR31E1; XAP104; H105e3; Sterol-4-Alpha-Carboxylate 3-Dehydrogenase, Decarboxylating; Short Chain Dehydrogenase/Reductase Family 31E, Member 1; Protein H105e3; EC 1.1.1.170; NAD(P) Dependent Steroid Dehydrogenase-Like Protein Transcript; Epididymis Secretory Sperm Binding Protein; H105E3
About Gene Symbol
NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like) is a metabolic dehydrogenase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular me
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