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KD-Validated Anti-NOTCH1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2220
Application
WB, FC
Uniprot Id
P46531
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-notch receptor 1 antibody (Cat#ABG2220). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-notch receptor 1 antibody (Cat#ABG2220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-notch receptor 1 antibody (Cat#ABG2220). Notch receptor 1 expression in wild-type (WT) and notch receptor 1 (NOTCH1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-notch receptor 1 antibody (Cat#ABG2220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Notch receptor 1 expression in C2C12 cells using anti-Notch receptor 1 antibody (Cat#ABG2220, 1:2,000). Green, isotype control; red, Notch receptor 1.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2220-100 3 days 100 µL $499.00
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ABG2220-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NOTCH1 Rabbit Monoclonal Antibody
Accession (Primary)P46531
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB425
Immunogen
A synthesized peptide derived from human Notch 1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 125 kDa; observed, 125 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P46531 )

Gene NameNOTCH1
Other Gene Names
NOTCH1; Notch Receptor 1; Notch 1; TAN1; Translocation-Associated Notch Protein TAN-1; Neurogenic Locus Notch Homolog Protein 1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1, Translocation-Associated (Drosophila); Notch Homolog 1, Translocation-Associated; EC 3.4.21.68; EC 2.1.2.11; AOVD1; AOS5
About Gene Symbol
NOTCH1 (Notch Receptor 1) is a membrane receptor involved in receptor-mediated signaling. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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