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[KO]Eph receptor B4/HTK Rabbit mAb

Catalog #: ABZ4671
Application: WB, IHC-P
Uniprot Id: P54760
Reactivity: Human, Mouse
Isotype: IgG

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Western blot analysis of Ephrin Receptor B4 in HeLa(WT) and Ephrin Receptor B4-Knockout HeLa(KO) using Ephrin Receptor B4 antibody.
Western blot analysis of Eph receptor B4/HTK in MEF lysates using Ephrin Receptor B4 antibody.
Western blot analysis of Eph receptor B4/HTK in Hela, A549, C6 lysates using Eph receptor B4/HTK antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Eph receptor B4/HTK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Datasheet
Citations
Reviews

Species Reactivity
Product Overview
Name	[KO]Eph receptor B4/HTK Rabbit mAb
Accession (Primary)	P54760
Host Species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Reacts With	Human, Mouse
Calculated MW
Calculated MW: 108 kDa; Observed MW: 135 kDa
Buffer System
Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Recommended Dilution
WB-1:500-1:1000; IHC-1:200-1:1000
Purification
Affinity Purified
Conjugation
Unconjugated
Modification
Unmodified
Form
Liquid
Background
EPH receptor B4 (EphB4), with 987-amino acid protein (about 108kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptor tyrosine kinases and their ligands, the ephrins, regulate numerous biological processes in developing and adult tissues and have been implicated in cancer progression and in pathological forms of angiogenesis. EphB4 acts as a negative regulator of blood vessel branching and vascular network formation, switching the vascularization program from sprouting angiogenesis to circumferential vessel growth. EphB4 and its ligand ephrinB2 express in several kinds of tumor cells and correlate with tumorigenesis. EphB4 is thus a potential candidate as a predictor of disease outcome in several kinds of tumor and as target for novel therapy.
Target Information: ( P54760 )
Name	EPHB4
Gene ID	2050
Other Names
EPHB4; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11; Ephb4; EPHB4_HUMAN; Ephrin receptor EphB4;

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