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KD-Validated Anti-ACADVL Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1225
Application
WB, FC, ICC
Uniprot Id
P49748
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ACADVL antibody (Cat#ABG1225). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACADVL antibody (Cat#ABG1225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ACADVL antibody (Cat#ABG1225). ACADVL expression in wild type (WT) and ACADVL shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ACADVL antibody (Cat#ABG1225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ACADVL expression in HepG2 cells using ACADVL antibody (Cat#ABG1225, 1:2,000). Green, isotype control; red, ACADVL.
  • Immunocytochemical staining of HepG2 cells with ACADVL antibody (Cat#ABG1225, 1:1,000). Nuclei were stained blue with DAPI; ACADVL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1225-100 3 days 100 µL $499.00
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ABG1225-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ACADVL Rabbit Monoclonal Antibody
Accession (Primary)P49748
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3615
Immunogen
A synthesized peptide derived from human ACADVL/VLCAD
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 70 kDa , observed, 65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P49748 )

Gene NameACADVL
Other Gene Names
ACADVL; Acyl-CoA Dehydrogenase Very Long Chain; VLCAD; LCACD; ACAD6; Very Long-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, Very Long Chain; EC 1.3.8.9; EC 1.3.99
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