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KD-Validated Anti-AP2S1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1149
Application
WB, FC, ICC
Uniprot Id
P53680
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-AP2S1 antibody (Cat#ABG1149). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP2S1 antibody (Cat#ABG1149, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-AP2S1 antibody (Cat#ABG1149). AP2S1 expression in wild type (WT) and AP2S1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-AP2S1 antibody (Cat#ABG1149 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of AP2S1 expression in C2C12 cells using AP2S1 antibody (Cat#ABG1149, 1:2,000). Green, isotype control; red, AP2S1.
  • Immunocytochemical staining of C2C12 cells with AP2S1 antibody (Cat#ABG1149, 1:1,000). Nuclei were stained blue with DAPI; AP2S1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1149-100 3 days 100 µL $499.00
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ABG1149-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AP2S1 Rabbit Monoclonal Antibody
Accession (Primary)P53680
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2980
Immunogen
A synthesized peptide derived from AP2S1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 17 kDa , observed, 17 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P53680 )

Gene NameAP2S1
Other Gene Names
AP2S1; Adaptor Related Protein Complex 2 Subunit Sigma 1; CLAPS2; FBH3; Adaptor Related Protein Complex 2 Sigma 1 Subunit; Clathrin Assembly Protein 2 Sigma Small Chain; Plasma Membrane Adaptor AP-2 17 KDa Protein; Adaptor Protein Complex AP-2 Subunit Sigma; Clathrin Coat-Associated Protein AP17; Clathrin Coat Assembly Protein AP17; AP-2 Complex Subunit Sigma; HA2 17 KDa Subunit; Sigma2-Adaptin; Sigma-2; FBHOk; AP17; HHC3; Clathrin-Associated/Assembly/Adaptor Protein, Small 2 (17kD); Adaptor-Related Protein Complex 2 Subunit Sigma; Hypocalciuric Hypercalcemia 3 (Oklahoma Type); FBHOK
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