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KD-Validated Anti-Acetyl-CoA carboxylase alpha Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1536
Application
WB, FC, ICC
Uniprot Id
Q13085
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Acetyl-CoA carboxylase alpha antibody (Cat#ABG1536). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA carboxylase alpha antibody (Cat#ABG1536, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Acetyl-CoA carboxylase α antibody (Cat#ABG1536). Acetyl-CoA carboxylase α expression in wild type (WT) and Acetyl-CoA carboxylase α shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA carboxylase α antibody (Cat#ABG1536, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Acetyl-CoA carboxylase alpha expression in HepG2 cells using Acetyl-CoA carboxylase alpha antibody (Cat#ABG1536, 1:2,000). Green, isotype control; red, Acetyl-CoA carboxylase alpha.
  • Immunocytochemical staining of HepG2 cells with anti-Acetyl-CoA carboxylase alpha antibody (Cat#ABG1536, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA carboxylase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1536-100 3 days 100 µL $499.00
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ABG1536-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Acetyl-CoA carboxylase alpha Rabbit Monoclonal Antibody
Accession (Primary)Q13085
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB5645
Immunogen
A synthesized peptide derived from human Acetyl Coenzyme A Carboxylase
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 266 kDa , observed, 266 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q13085 )

Gene NameACACA
Other Gene Names
ACACA; Acetyl-CoA Carboxylase Alpha; ACC1; ACCA; Acetyl-Coenzyme A Carboxylase Alpha; Acetyl-CoA Carboxylase 1; ACC-Alpha; HACC1; ACAC; ACACalpha; ACCalpha; Acac1; ACC; EC 6.4.1.2; ACACALPHA; ACC-ALPHA; ACCALPHA; ACACAD; ACAC1
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