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KD-Validated Anti-Actin like 6A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2348
Application
WB, FC, ICC
Uniprot Id
O96019
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Actin like 6A antibody (Cat#ABG2348). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Actin like 6A antibody (Cat#ABG2348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Actin like 6A antibody (Cat#ABG2348). Actin like 6A expression in wild type (WT) and actin like 6A shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Actin like 6A antibody (Cat#ABG2348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Actin like 6A expression in HepG2 cells using Actin like 6A antibody (Cat#ABG2348, 1:2,000). Green, isotype control; red, Actin like 6A.
  • Immunocytochemical staining of HT-1080 cells with Actin like 6A antibody (Cat#ABG2348, 1:1,000). Nuclei were stained blue with DAPI; Actin like 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2348-100 3 days 100 µL $499.00
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ABG2348-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Actin like 6A Rabbit Monoclonal Antibody
Accession (Primary)O96019
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1155
Immunogen
A synthesized peptide derived from human ACTL6A
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 4 7 kDa ; Observed, 4 7 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry(FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O96019 )

Gene NameACTL6A
Other Gene Names
ACTL6A; Actin Like 6A; BAF53A; INO80K; BRG1-Associated Factor 53A; INO80 Complex Subunit K; SMARCN1; 53 KDa BRG1-Associated Factor A; Actin-Related Protein Baf53a; BAF Complex 53 KDa Subunit; Actin-Related Protein; Actin-Like Protein 6A; ArpNbeta; ACTL6; BAF53; Arp4; HArpN Beta; ARPN-BETA; Baf53a; Actl6; ARP4
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