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KD-Validated Anti-Aminopeptidase A / CD249 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1518
Application
WB, ICC
Uniprot Id
Q07075
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518). Aminopeptidase A / CD249 expression in wild type (WT) and Aminopeptidase A / CD249 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:1,000). Nuclei were stained blue with DAPI; Aminopeptidase A / CD249 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1518-100 3 days 100 µL $499.00
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ABG1518-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Aminopeptidase A / CD249 Rabbit Monoclonal Antibody
Accession (Primary)Q07075
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB5325
Immunogen
A synthesized peptide derived from human Aminopeptidase A / CD249
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 109 kDa, observed, 250kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q07075 )

Gene NameENPEP
Other Gene Names
ENPEP; Glutamyl Aminopeptidase; CD249; Differentiation Antigen Gp160; Aminopeptidase A; EC 3.4.11.7; Gp160; AP-A; EAP; Glutamyl Aminopeptidase (Aminopeptidase A); CD249 Antigen; EC 3.4.11; GP160; APA
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ADMEbio Team

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